stem cell factor mscf Search Results


mouse stem cell factor scf  (Gemini Bio)
90
Gemini Bio mouse stem cell factor scf
Mouse Stem Cell Factor Scf, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse stem cell factor scf/product/Gemini Bio
Average 90 stars, based on 1 article reviews
mouse stem cell factor scf - by Bioz Stars, 2026-05
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30 ng/ml murine stem cell factor (scf)  (PeproTech)
90
PeproTech 30 ng/ml murine stem cell factor (scf)
(MK) differentiation from R26M2K2 iPSCs. (A) Differentiation scheme for MK and platelet generation from murine iPSCs. After retroviral transduction, iPSCs were differentiated into EBs by orbital shaking for 6 days (day −6); at day 5, hematopoietic differentiation was enforced by addition of <t>SCF</t> <t>and</t> <t>interleukin‐3.</t> At day 1, CD41 positive early hematopoietic progenitors were selected by magnetic‐associated cell sorting and seeded onto OP9 feeder cells with thrombopoietin and stem cell factor and induced by doxycycline. After day 14, MK morphology and surface marker expression were analyzed. (B) CD41 expression on early hematopoietic progenitors after EB dissociation. (n = 3‐9, mean ± SD, no differences by one‐way analysis of variance ANOVA with Dunnett’s multiple comparison test). (C) Representative flow cytometry plots of cells after embryoid body (EB) dissociation showing expression of CD41 that is not coexpressed with the pluripotency marker Stage‐specific embryonic antigen 1 (left plot). No GFP expression could be detected at that time, (right plot). (D) Green fluorescent protein (GFP) expression in EBs measured at the day of dissociation in the uninduced state. GFP expression in MKs after 2‐week induction with doxycycline. No expression after 1‐week withdrawal of doxycycline during further cultivation of MKs (GATA‐1 transduced MKs are shown, mean ± SD, n = 3‐5) (E) Percentage of CD41 expression and CD41/CD42d double positive cells at days 14 of differentiation on hematopoietic cell differentiated on OP9 feeders, (n = 3‐8, mean ± SD, one‐way ANOVA with Dunnett’s multiple comparison test, *** P < .00; Mann‐Whitney test, * P = .006). (F) Ratio of CD42d to CD41 expression as a measure of differentiation. (G) Quantification of CD41 + MK at day 14 ((F) and (G): n = 3‐8, mean ± SD, Mann‐Whitney test)
30 Ng/Ml Murine Stem Cell Factor (Scf), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/30 ng/ml murine stem cell factor (scf)/product/PeproTech
Average 90 stars, based on 1 article reviews
30 ng/ml murine stem cell factor (scf) - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
stem cell factor mscf  (PeproTech)
90
PeproTech stem cell factor mscf
(MK) differentiation from R26M2K2 iPSCs. (A) Differentiation scheme for MK and platelet generation from murine iPSCs. After retroviral transduction, iPSCs were differentiated into EBs by orbital shaking for 6 days (day −6); at day 5, hematopoietic differentiation was enforced by addition of <t>SCF</t> <t>and</t> <t>interleukin‐3.</t> At day 1, CD41 positive early hematopoietic progenitors were selected by magnetic‐associated cell sorting and seeded onto OP9 feeder cells with thrombopoietin and stem cell factor and induced by doxycycline. After day 14, MK morphology and surface marker expression were analyzed. (B) CD41 expression on early hematopoietic progenitors after EB dissociation. (n = 3‐9, mean ± SD, no differences by one‐way analysis of variance ANOVA with Dunnett’s multiple comparison test). (C) Representative flow cytometry plots of cells after embryoid body (EB) dissociation showing expression of CD41 that is not coexpressed with the pluripotency marker Stage‐specific embryonic antigen 1 (left plot). No GFP expression could be detected at that time, (right plot). (D) Green fluorescent protein (GFP) expression in EBs measured at the day of dissociation in the uninduced state. GFP expression in MKs after 2‐week induction with doxycycline. No expression after 1‐week withdrawal of doxycycline during further cultivation of MKs (GATA‐1 transduced MKs are shown, mean ± SD, n = 3‐5) (E) Percentage of CD41 expression and CD41/CD42d double positive cells at days 14 of differentiation on hematopoietic cell differentiated on OP9 feeders, (n = 3‐8, mean ± SD, one‐way ANOVA with Dunnett’s multiple comparison test, *** P < .00; Mann‐Whitney test, * P = .006). (F) Ratio of CD42d to CD41 expression as a measure of differentiation. (G) Quantification of CD41 + MK at day 14 ((F) and (G): n = 3‐8, mean ± SD, Mann‐Whitney test)
Stem Cell Factor Mscf, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stem cell factor mscf/product/PeproTech
Average 90 stars, based on 1 article reviews
stem cell factor mscf - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


(MK) differentiation from R26M2K2 iPSCs. (A) Differentiation scheme for MK and platelet generation from murine iPSCs. After retroviral transduction, iPSCs were differentiated into EBs by orbital shaking for 6 days (day −6); at day 5, hematopoietic differentiation was enforced by addition of SCF and interleukin‐3. At day 1, CD41 positive early hematopoietic progenitors were selected by magnetic‐associated cell sorting and seeded onto OP9 feeder cells with thrombopoietin and stem cell factor and induced by doxycycline. After day 14, MK morphology and surface marker expression were analyzed. (B) CD41 expression on early hematopoietic progenitors after EB dissociation. (n = 3‐9, mean ± SD, no differences by one‐way analysis of variance ANOVA with Dunnett’s multiple comparison test). (C) Representative flow cytometry plots of cells after embryoid body (EB) dissociation showing expression of CD41 that is not coexpressed with the pluripotency marker Stage‐specific embryonic antigen 1 (left plot). No GFP expression could be detected at that time, (right plot). (D) Green fluorescent protein (GFP) expression in EBs measured at the day of dissociation in the uninduced state. GFP expression in MKs after 2‐week induction with doxycycline. No expression after 1‐week withdrawal of doxycycline during further cultivation of MKs (GATA‐1 transduced MKs are shown, mean ± SD, n = 3‐5) (E) Percentage of CD41 expression and CD41/CD42d double positive cells at days 14 of differentiation on hematopoietic cell differentiated on OP9 feeders, (n = 3‐8, mean ± SD, one‐way ANOVA with Dunnett’s multiple comparison test, *** P < .00; Mann‐Whitney test, * P = .006). (F) Ratio of CD42d to CD41 expression as a measure of differentiation. (G) Quantification of CD41 + MK at day 14 ((F) and (G): n = 3‐8, mean ± SD, Mann‐Whitney test)

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Forming megakaryocytes from murine induced pluripotent stem cells by the inducible overexpression of supporting factors

doi: 10.1002/rth2.12453

Figure Lengend Snippet: (MK) differentiation from R26M2K2 iPSCs. (A) Differentiation scheme for MK and platelet generation from murine iPSCs. After retroviral transduction, iPSCs were differentiated into EBs by orbital shaking for 6 days (day −6); at day 5, hematopoietic differentiation was enforced by addition of SCF and interleukin‐3. At day 1, CD41 positive early hematopoietic progenitors were selected by magnetic‐associated cell sorting and seeded onto OP9 feeder cells with thrombopoietin and stem cell factor and induced by doxycycline. After day 14, MK morphology and surface marker expression were analyzed. (B) CD41 expression on early hematopoietic progenitors after EB dissociation. (n = 3‐9, mean ± SD, no differences by one‐way analysis of variance ANOVA with Dunnett’s multiple comparison test). (C) Representative flow cytometry plots of cells after embryoid body (EB) dissociation showing expression of CD41 that is not coexpressed with the pluripotency marker Stage‐specific embryonic antigen 1 (left plot). No GFP expression could be detected at that time, (right plot). (D) Green fluorescent protein (GFP) expression in EBs measured at the day of dissociation in the uninduced state. GFP expression in MKs after 2‐week induction with doxycycline. No expression after 1‐week withdrawal of doxycycline during further cultivation of MKs (GATA‐1 transduced MKs are shown, mean ± SD, n = 3‐5) (E) Percentage of CD41 expression and CD41/CD42d double positive cells at days 14 of differentiation on hematopoietic cell differentiated on OP9 feeders, (n = 3‐8, mean ± SD, one‐way ANOVA with Dunnett’s multiple comparison test, *** P < .00; Mann‐Whitney test, * P = .006). (F) Ratio of CD42d to CD41 expression as a measure of differentiation. (G) Quantification of CD41 + MK at day 14 ((F) and (G): n = 3‐8, mean ± SD, Mann‐Whitney test)

Article Snippet: At day 5 of EB formation, medium was supplemented with 10 ng/mL murine interleukin‐3 and 30 ng/mL murine stem cell factor (SCF) (PeproTech, Rocky Hill, NJ, USA).

Techniques: Transduction, FACS, Marker, Expressing, Flow Cytometry, MANN-WHITNEY